解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The membrane-bound transcription factor ATF6α plays a cytoprotective role in the unfolded protein response (UPR), required for cells to survive ER stress. Activation of ATF6α promotes cell survival in cancer models. We used cell-based screens to discover and develop Ceapins, a class of pyrazole amides, that block ATF6...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11878
更新日期:2016-07-20 00:00:00
abstract::We recently reported that the C2AB portion of Synaptotagmin 1 (Syt1) could self-assemble into Ca(2+)-sensitive ring-like oligomers on membranes, which could potentially regulate neurotransmitter release. Here we report that analogous ring-like oligomers assemble from the C2AB domains of other Syt isoforms (Syt2, Syt7,...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17262
更新日期:2016-07-19 00:00:00
abstract::Experiments in which monkeys have to select or estimate the location of a target are revealing more about the role of the dorsal premotor cortex in decision making. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.18721
更新日期:2016-07-15 00:00:00
abstract::We determined whether the mutations found in ovarian cancers could be identified in the patients' ovarian cyst fluids. Tumor-specific mutations were detectable in the cyst fluids of 19 of 23 (83%) borderline tumors, 10 of 13 (77%) type I cancers, and 18 of 18 (100%) type II cancers. In contrast, no mutations were foun...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15175
更新日期:2016-07-15 00:00:00
abstract::Genetic programming and neural activity drive synaptic remodeling in developing neural circuits, but the molecular components that link these pathways are poorly understood. Here we show that the C. elegans Degenerin/Epithelial Sodium Channel (DEG/ENaC) protein, UNC-8, is transcriptionally controlled to function as a ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14599
更新日期:2016-07-12 00:00:00
abstract::The structure of fitness landscapes is critical for understanding adaptive protein evolution. Previous empirical studies on fitness landscapes were confined to either the neighborhood around the wild type sequence, involving mostly single and double mutants, or a combinatorially complete subgraph involving only two am...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16965
更新日期:2016-07-08 00:00:00
abstract::Increasing evidence highlights the important roles of microRNAs in mediating p53's tumor suppression functions. Here, we report miR-139-5p as another new p53 microRNA target. p53 induced the transcription of miR-139-5p, which in turn suppressed the protein levels of phosphodiesterase 4D (PDE4D), an oncogenic protein i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15978
更新日期:2016-07-07 00:00:00
abstract::Metabolic network rewiring is the rerouting of metabolism through the use of alternate enzymes to adjust pathway flux and accomplish specific anabolic or catabolic objectives. Here, we report the first characterization of two parallel pathways for the breakdown of the short chain fatty acid propionate in Caenorhabditi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17670
更新日期:2016-07-06 00:00:00
abstract::Mutations in PROP1 are the most common cause of hypopituitarism in humans; therefore, unraveling its mechanism of action is highly relevant from a therapeutic perspective. Our current understanding of the role of PROP1 in the pituitary gland is limited to the repression and activation of the pituitary transcription fa...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14470
更新日期:2016-06-28 00:00:00
abstract::Vesicle fusion is mediated by an assembly of SNARE proteins between opposing membranes, but it is unknown whether transmembrane domains (TMDs) of SNARE proteins serve mechanistic functions that go beyond passive anchoring of the force-generating SNAREpin to the fusing membranes. Here, we show that conformational flexi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.17571
更新日期:2016-06-25 00:00:00
abstract::Myosins play essential roles in the development and function of auditory organs and multiple myosin genes are associated with hereditary forms of deafness. Using a forward genetic screen in Drosophila, we identified an E3 ligase, Ubr3, as an essential gene for auditory organ development. Ubr3 negatively regulates the ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15258
更新日期:2016-06-22 00:00:00
abstract::Goal-directed behavior involves distributed neuronal circuits in the mammalian brain, including diverse regions of neocortex. However, the cellular basis of long-range cortico-cortical signaling during goal-directed behavior is poorly understood. Here, we recorded membrane potential of excitatory layer 2/3 pyramidal n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15798
更新日期:2016-06-21 00:00:00
abstract::Recent work draws attention to community-community encounters ('coalescence') as likely an important factor shaping natural ecosystems. This work builds on MacArthur's classic model of competitive coexistence to investigate such community-level competition in a minimal theoretical setting. It is shown that the ability...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15747
更新日期:2016-06-16 00:00:00
abstract::Neurons coding for head-direction are crucial for spatial navigation. Here we explored the cellular basis of head-direction coding in the rat dorsal presubiculum (PreS). We found that layer2 is composed of two principal cell populations (calbindin-positive and calbindin-negative neurons) which targeted the contralater...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14592
更新日期:2016-06-10 00:00:00
abstract::A broadly known method to stimulate the growth potential of axons is to elevate intracellular levels of cAMP, however the cellular pathway(s) that mediate this are not known. Here we identify the Dual Leucine-zipper Kinase (DLK, Wnd in Drosophila) as a critical target and effector of cAMP in injured axons. DLK/Wnd is ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14048
更新日期:2016-06-07 00:00:00
abstract::Complexin regulates spontaneous and activates Ca(2+)-triggered neurotransmitter release, yet the molecular mechanisms are still unclear. Here we performed single molecule fluorescence resonance energy transfer experiments and uncovered two conformations of complexin-1 bound to the ternary SNARE complex. In the cis con...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.16886
更新日期:2016-06-02 00:00:00
abstract::The spliceosome is a complex machine composed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from pre-mRNAs. After assembly the spliceosome is activated for catalysis by rearrangement of subunits to form an active site. How this rearrangement is coordinated is not well-underst...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14166
更新日期:2016-05-31 00:00:00
abstract::Gap junctions are widely distributed in the brains across species and play essential roles in neural information processing. However, the role of gap junctions in insect cognition remains poorly understood. Using a flight simulator paradigm and genetic tools, we found that gap junctions are present in Drosophila Kenyo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13238
更新日期:2016-05-24 00:00:00
abstract::Islet amyloidosis by IAPP contributes to pancreatic β-cell death in diabetes, but the nature of toxic IAPP species remains elusive. Using concurrent time-resolved biophysical and biological measurements, we define the toxic species produced during IAPP amyloid formation and link their properties to induction of rat IN...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12977
更新日期:2016-05-23 00:00:00
abstract::A spring-loaded mechanism can explain the activation process for a protein that has a crucial role in maintaining the genomic integrity of immature eggs cells. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.17394
更新日期:2016-05-23 00:00:00
abstract::Advanced microscopy and labeling techniques reveal that bacteria localize mRNAs within their cells in a similar way to eukaryotes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.16598
更新日期:2016-05-20 00:00:00
abstract::PKMζ is a persistently active PKC isoform proposed to maintain late-LTP and long-term memory. But late-LTP and memory are maintained without PKMζ in PKMζ-null mice. Two hypotheses can account for these findings. First, PKMζ is unimportant for LTP or memory. Second, PKMζ is essential for late-LTP and long-term memory i...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14846
更新日期:2016-05-17 00:00:00
abstract::Burkholderia thailandensis uses acyl-homoserine lactone-mediated quorum sensing systems to regulate hundreds of genes. Here we show that cell-cell contact-dependent type VI secretion (T6S) toxin-immunity systems are among those activated by quorum sensing in B. thailandensis. We also demonstrate that T6S is required t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14712
更新日期:2016-05-16 00:00:00
abstract::Certain neuron types fire spontaneously at high rates, an ability that is crucial for their function in brain circuits. The spontaneously active GABAergic neurons of the substantia nigra pars reticulata (SNr), a major output of the basal ganglia, provide tonic inhibition of downstream brain areas. A depolarizing 'leak...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15271
更新日期:2016-05-13 00:00:00
abstract::Mood and anxiety disorders are the most prevalent psychiatric conditions and are exacerbated by stress. Recent studies have suggested cyclooxygenase-2 (COX-2) inhibition could represent a novel treatment approach or augmentation strategy for affective disorders including anxiety disorders and major depression. We show...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14137
更新日期:2016-05-10 00:00:00
abstract::The axolotl can regenerate multiple organs, including the brain. It remains, however, unclear whether neuronal diversity, intricate tissue architecture, and axonal connectivity can be regenerated; yet, this is critical for recovery of function and a central aim of cell replacement strategies in the mammalian central n...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13998
更新日期:2016-05-09 00:00:00
abstract::Duplicating chromosomes once each cell cycle produces sister chromatid pairs, which separate accurately at anaphase. In contrast, reduplicating chromosomes without separation frequently produces polytene chromosomes, a barrier to accurate mitosis. Chromosome reduplication occurs in many contexts, including: polytene t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.15204
更新日期:2016-05-09 00:00:00
abstract::The CRISPR-Cas9 bacterial surveillance system has become a versatile tool for genome editing and gene regulation in eukaryotic cells, yet how CRISPR-Cas9 contends with the barriers presented by eukaryotic chromatin is poorly understood. Here we investigate how the smallest unit of chromatin, a nucleosome, constrains t...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13450
更新日期:2016-04-28 00:00:00
abstract::Translation is a core cellular process carried out by a highly conserved macromolecular machine, the ribosome. There has been remarkable evolutionary adaptation of this machine through the addition of eukaryote-specific ribosomal proteins whose individual effects on ribosome function are largely unknown. Here we show ...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11154
更新日期:2016-04-27 00:00:00
abstract::Establishing a link between the nonsense-mediated decay pathway and a gene associated with programmed cell death could explain why this pathway is essential in most, but not all, eukaryotes. ...
journal_title:eLife
pub_type: 评论,杂志文章
doi:10.7554/eLife.16076
更新日期:2016-04-27 00:00:00
abstract::The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities. The complex regulates the higher-order structure of chromatin, and has important roles in the regulation of gene expression, DNA damage repair and cell differentiation. H...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13941
更新日期:2016-04-21 00:00:00
abstract::The roles of long non-coding RNAs (lncRNAs) in regulating cancer and stem cells are being increasingly appreciated. Its diverse mechanisms provide the regulatory network with a bigger repertoire to increase complexity. Here we report a novel LncRNA, Lnc34a, that is enriched in colon cancer stem cells (CCSCs) and initi...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14620
更新日期:2016-04-14 00:00:00
abstract::The permeability barrier of nuclear pore complexes (NPCs) controls bulk nucleocytoplasmic exchange. It consists of nucleoporin domains rich in phenylalanine-glycine motifs (FG domains). As a bottom-up nanoscale model for the permeability barrier, we have used planar films produced with three different end-grafted FG d...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14119
更新日期:2016-04-08 00:00:00
abstract::Huntington disease (HD) is a neurological disorder caused by polyglutamine expansions in mutated Huntingtin (mHtt) proteins, rendering them prone to form inclusion bodies (IB). We report that in yeast, such IB formation is a factor-dependent process subjected to age-related decline. A genome-wide, high-content imaging...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11792
更新日期:2016-04-01 00:00:00
abstract::Progression through the mitotic cell cycle requires periodic regulation of gene function at the levels of transcription, translation, protein-protein interactions, post-translational modification and degradation. However, the role of alternative splicing (AS) in the temporal control of cell cycle is not well understoo...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.10288
更新日期:2016-03-25 00:00:00
abstract::mTOR inhibition is beneficial in neurodegenerative disease models and its effects are often attributable to the modulation of autophagy and anti-apoptosis. Here, we report a neglected but important bioenergetic effect of mTOR inhibition in neurons. mTOR inhibition by rapamycin significantly preserves neuronal ATP leve...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.13378
更新日期:2016-03-23 00:00:00
abstract::The organization and biophysical properties of the cytosol implicitly govern molecular interactions within cells. However, little is known about mechanisms by which cells regulate cytosolic properties and intracellular diffusion rates. Here, we demonstrate that the intracellular environment of budding yeast undertakes...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.09376
更新日期:2016-03-22 00:00:00
abstract::Nucleolar protein interacting with the FHA domain of pKi-67 (NIFK) is a Ki-67-interacting protein. However, its precise function in cancer remains largely uninvestigated. Here we show the clinical significance and metastatic mechanism of NIFK in lung cancer. NIFK expression is clinically associated with poor prognosis...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.11288
更新日期:2016-03-17 00:00:00
abstract::Recent findings indicate a high level of specialization at the level of microcircuits and cell populations within brain structures with regards to the control of fear and anxiety. The hippocampus, however, has been treated as a unitary structure in anxiety and fear research despite mounting evidence that different hip...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.14120
更新日期:2016-03-14 00:00:00
abstract::To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away from biotin after enrichment, and a spacer arm that can be label...
journal_title:eLife
pub_type: 杂志文章
doi:10.7554/eLife.12509
更新日期:2016-03-08 00:00:00